Purdue University Graduate School
CAR T cell Thesis XRCardona.pdf (1.56 MB)

Canine CAR T cell therapy for solid tumors

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posted on 2023-04-20, 20:57 authored by Xavier E Ramos CardonaXavier E Ramos Cardona


Adoptive cell transfer of chimeric antigen receptors (CAR) T cells has successfully targeted hematological malignancies in human patients. However, unpredicted side effects experienced after injection of the CAR T cells suggests the need for an optimal predictive preclinical animal model. Dogs have intact immune systems and develop solid tumors spontaneously with similar morphology and genetics to humans. I hypothesize that generating CAR T cells for dogs will closely mimic human patients' outcomes, thus providing new understandings of the safety of this immunotherapy. In addition to the dog as a preclinical model, we propose using a universal CAR T cell to overcome various tumor-related immunosuppressive challenges and control the killing of the target cells. To achieve this, we established methods for activating and expanding canine T cells to a clinically relevant scale. Then, we expressed a second-generation anti-FITC-8-41BB-ζ CAR T cell via lentiviral transduction. In the presence of the correct low-molecular-weight bispecific adapter, we showed in-vitro CAR-mediated function. Our results proved that it is feasible to generate functional canine anti-FITC-8-BB-ζ CAR T cells for therapy.


Degree Type

  • Master of Science


  • Comparative Pathobiology

Campus location

  • West Lafayette

Advisor/Supervisor/Committee Chair

Sulma Mohammed

Additional Committee Member 2

Suresh K. Mittal

Additional Committee Member 3

GuangJun Zhang

Additional Committee Member 4

Phillip Low

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