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Development of Biodynamic Imaging for Phenotypic Profiling of Living Tissue

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thesis
posted on 2022-12-09, 15:20 authored by Zhen HuaZhen Hua

Biodynamic imaging (BDI) is a high-content optical imaging technology based on Fourier-domain digital holography and Doppler spectroscopy of intracellular dynamics. There are three main functions of the BDI technique, which are optical coherence imaging (OCI), motility contrast imaging (MCI) and tissue dynamics spectroscopy (TDS). OCI is related to en face optical coherence tomography (OCT) using partially coherent speckle generated by broad-area illumination with coherence detection through digital holography. MCI provides noninvasive functional imaging by treating intracellular motility as an endogenous dynamic imaging contrast agent. TDS produces broad-band Doppler fluctuation power spectra that contain the ensemble of all intracellular motions by collecting and extracting depth-resolved quasi-elastic dynamic light scattering from inside multicellular living tissue. This thesis presents the development and applications of BDI systems. Doppler spectral clustering analysis is demonstrated when comparing fresh canine lymphoma biopsies and their corresponding flash-Frozen samples. Doppler spectral phenotyping analysis is used to identify a non-predictive phenotype of TDS that shows a systemic red-shift of frequencies. Doppler spectral shift analysis is used to monitor bacterial infection of living tissue. 

Funding

NSF CBET-2200186

History

Degree Type

  • Doctor of Philosophy

Department

  • Physics and Astronomy

Campus location

  • West Lafayette

Advisor/Supervisor/Committee Chair

David Nolte

Additional Committee Member 2

Kenneth Ritchie

Additional Committee Member 3

Martin Kruczenski

Additional Committee Member 4

Oana Malis

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