Purdue University Graduate School
Azam Shafieenezhad-thesis.pdf (11.85 MB)

Interactions of Neuromodulators with Lipid Bilayers Studied by Scattering and Spectroscopy Methods

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posted on 2022-11-28, 19:47 authored by Azam ShafieenezhadAzam Shafieenezhad

This work studies the effect of dopamine (DA) and adenosine triphosphate (ATP) on lipid membranes using a number of complementary experimental methods. These methods include Dynamic Light Scattering to measure electrostatic surface potentials, solid-state Nuclear Magnetic Resonance to measure the degree of lipid acyl chain order, Electron Paramagnetic Resonance to measure changes in membrane viscosity, and X-ray diffuse scattering to measure structural and material parameters of lipid bilayers. It is shown that both DA and ATP have a measurable affinity to the lipid-water interface even in the absence of specialized biological receptors. These results are important for understanding the function of DA and ATP in cellular processes.


Degree Type

  • Doctor of Philosophy


  • Physics and Astronomy

Campus location

  • West Lafayette

Advisor/Supervisor/Committee Chair

Horia I. Petrache

Additional Committee Member 2

Stephen R. Wassall

Additional Committee Member 3

Gautam Vemuri

Additional Committee Member 4

Ruihua Cheng

Additional Committee Member 5

Yogesh N. Joglekar

Additional Committee Member 6

Kenneth P. Ritchie