NANO-DESI IMAGING OF EICOSANOIDS IN MOUSE KIDNEY TISSUE USING SELECTED ION MONITORING
thesisposted on 2021-05-02, 22:00 authored by Courtney Dale HuffstutlerCourtney Dale Huffstutler
Nano-DESI Mass spectrometry imaging of eicosanoids in mouse kidney tissue using selected ion monitoring. Nano-DESI mass spectrometry imaging (MSI) is a technique for label-free spatial and molecular characterization of surfaces and biological samples. Eicosanoids are lipid mediators derived from eicosapolyenoic acid- products of arachidonic acid oxidation. Eicosanoids have been of interest to the medical field for many years. Major focus on this lipid class came from the development of nonsteroidal anti-inflammatory drugs (NSAIDs), some of these including aspirin, naproxen, ibuprofen, and acetaminophen work by blocking either the formation or the effects of eicosanoids. These lipids also play important roles in various body functions (cardiovascular, renal, gastrointestinal, neuronal) and as mediators of inflammation, asthma, fever, pain, hypertension, and stroke. Typically, eicosanoids occur in subnanomolar concentrations, despite their high level of bioactivity, which makes them significantly more difficult to analyze via direct mass spectrometry. Here, selected ion monitoring (SIM) is used to increase the signal-to-noise of the identified eicosanoids compared to a broadband full scan mode.