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Development of Biofilms that Enable the Persistence of Listeria monocytogenes in Food Processing environments Despite Adequate Sanitation Procedures

thesis
posted on 2024-10-09, 16:50 authored by Jack Burnett

This thesis explores the complex relationship between Listeria monocytogenes and food processing environments (FPEs), focusing on the persistence mechanisms of this pathogen despite rigorous sanitation efforts. L. monocytogenes is a significant public health concern due to its association with high mortality rates in vulnerable populations. Recent studies, including the first chapter of this thesis, have highlighted the challenges in eradicating this pathogen from FPEs, suggesting that biofilms play a crucial role in its persistence. Despite various strategies and enhanced sanitation protocols, the eradication of L. monocytogenes remains elusive, underlining the need for a deeper understanding of its biofilm-associated resilience. Chapter two synthesizes findings from a systematic review and meta-analysis of studies examining the microbial communities on FPE surfaces through metagenomics, aiming to uncover patterns that might explain Listeria's long-term survival. In chapter three, the thesis delves into the nuanced role of biofilm composition and microbial diversity as factors enabling the persistence of Listeria monocytogenes in food processing environments (FPEs), despite comprehensive sanitation efforts. This section builds on the understanding that L. monocytogenes does not inherently form robust biofilms but thrives within complex microbial communities present on FPE surfaces. The findings presented in this thesis contribute to a more profound comprehension of L. monocytogenes' survival strategies, proposing a shift in focus towards the microbial ecosystem's metabolic interplays for the development of targeted control measures. This approach not only opens new avenues for research but also suggests practical implications for enhancing food safety protocols by considering the broader microbial dynamics at play within biofilms on FPE surfaces.

History

Degree Type

  • Doctor of Philosophy

Department

  • Food Science

Campus location

  • West Lafayette

Advisor/Supervisor/Committee Chair

Haley Oliver

Additional Committee Member 2

Stephen Lindemann

Additional Committee Member 3

Robert Pruitt

Additional Committee Member 4

Amanda Deering

Additional Committee Member 5

Dale Grinstead

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