Purdue University Graduate School
Saillant_MS_Thesis_Final.pdf (1.74 MB)

Evaluation of Prebiotic and Probiotic as Functional Feed Additives on Physiological and Immunological Parameters of Nile Tilapia, Oreochromis niloticus

Download (1.74 MB)
posted on 2019-06-10, 18:06 authored by Kenneth E SaillantKenneth E Saillant
Experiments were conducted to evaluate the ability of prebiotic and probiotic supplementation of commercial fish feed to improve the physiological, immunological, and growth responses of stressed Nile tilapia. To investigate these objectives, tilapia were divided in two major groups: control fish (fed regular commercial feed) and stressed fish (induced by dietary cortisol supplemented to regular commercial feed). Stressed fish were further divided into three sub-groups: stressed fish fed regular feed, stressed fish fed probiotic-supplemented feed, and stressed fish fed a mixture of prebiotic and probiotic supplemented feed. Fish were maintained and tested over an eight-week long experimental period. A variety of physiological, immunological, and growth parameters were measured over the course of the experimental period. These parameters include: serum cortisol, blood glucose, plasma protein, packed cell volume, hepato-somatic index (HSI), spleen-somatic index (SSI), lysozyme activity, feed conversion ratio (FCR), specific growth rate (SGR), protein efficiency ratio (PER), length gain, weight gain, length gain, and condition factor (K). The results of this study does not support the use of these specific prebiotic and probiotic as functional feed additives in Nile tilapia at the levels tested in this study. Further research is needed to determine which probiotic species are best suited for use in Nile tilapia and which prebiotic, when used in combination, will allow these probiotics to have maximum effect.


Degree Type

  • Master of Science


  • Biological Sciences

Campus location

  • Fort Wayne

Advisor/Supervisor/Committee Chair

Dr. Ahmed Mustafa

Additional Committee Member 2

Dr. Elliott J. Blumenthal

Additional Committee Member 3

Dr. Robert Gillespie